The usage of non-polar stationary section and polar cell phase assisted to individual these hydrophobic analytes. Given that this practice is reverse of normal phase, the term reversed stage is applied. This is similar to calling a ideal-handed ping pong player as ordinary and a left-handed ping pong player as reverse of authentic.HPLC is actually
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The schematic of an HPLC instrument generally includes a degasser, sampler, pumps, in addition to a detector. The sampler brings the sample combination in the mobile period stream which carries it in to the column. The pumps produce the specified stream and composition of the cell phase through the column. The detector generates a sign proportional
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